Journal: Neurophotonics

Article Title: Distribution of spine classes shows intra-neuronal dendritic heterogeneity in mouse cortex

doi: 10.1117/1.NPh.12.1.015001

Figure Lengend Snippet: Spine classes are inhomogeneously distributed on the dendrites. (a) Spines were categorized into four classes by hierarchical clustering of shape and length: upper panels—individual diameter profiles (gray) and average profile of each class (green). The total lengths of the profiles were scaled to the average spine length of the respective class; lower panels—representative spine examples. Scale bars, 1 μ m . (b) Relative abundance of each spine class on the dendrites, the distribution differs among dendrites. Numbers 1 to 4 refer to the four neurons, letters to the dendrites (see Fig. 1 ). Color code as in panel (a). (c) Pie charts showing which fraction of a specific spine class is found on each dendrite. (d) Pairwise Pearson’s chi-square tests confirm significant differences among the repartition of the classes on the dendrites within three of the four neurons. p values color coded: p ≤ 0.05 red, p ≤ 0.01 yellow, p ≤ 0.001 green, and p > 0.05 blue. All tests have been corrected for multiple comparisons. (e) Pairwise Pearson’s chi-square tests for all dendrites of all cells.

Article Snippet: We used Pearson’s chi-square test (Matlab’s function crosstab) to test for significant differences in class distribution on dendrites ( ) with a 5% significance level and corrected for multiple comparisons with the Bonferroni correction.

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